GCxGC
GCxGC (“GC by GC”), also known as Comprehensive Two-Dimensional Gas Chromatography, is a powerful analytic technique that utilizes two columns of differing phase selectivity connected by a modulation device. The set-up of GCxGC improves peak capacity, resolution, and detectability. On average, a GCxGC analysis has five times the sensitivity and gains three times the number of compound identifications of typical GC-MS runs.
As a technique that has been around for over 25 years, some may argue that the technique has been over-hyped and a complicated research tool that doesn’t fit into routine analytical laboratories. However, several routine validated GCxGC methods have demonstrated that the technique is accurate, precise, and robust. Research has shown the benefits of GCxGC in a wide variety of applications (e.g. environmental, metabolomics, petroleum, food safety, fragrance). The benefits include increased peak capacity (i.e. resolution), structured two-dimensional chromatograms (i.e. contour plots), and sensitivity enhancement.
The merits of GCxGC as a separation science are clear, but how will it benefit a routine laboratory? Multiple analyte classes can be combined into a single analysis to save instrument and sample preparation time. Manual review time for non-target screening methods can be reduced with the increased peak resolution of a GCxGC analysis by creating a better library match leading to faster, more confident peak identification. Sample characterization is improved with a GCxGC analysis which increases confidence in decisions based on analytical results. The extra resolution afforded by the GCxGC analysis allows the use of more economical detectors, like the flame ionization detector (FID) or electron capture detector (ECD).
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Instrument Portfolio
![Pegasus Two-Dimensional Gas Chromatograph](https://lecocorp.ams3.digitaloceanspaces.com/wp-content/uploads/20240102153326/pegasus-gcxgc.jpg)
GCxGC Time-of-Flight Mass Spectrometer
![Pegasus High Resolution two-dimensional gas chromatograph](https://lecocorp.ams3.digitaloceanspaces.com/wp-content/uploads/20240102153318/pegasus-highresolution-gcxgc.jpg)
High Resolution GCxGC-TOFMS
![QuadJet Mass Spectrometer](https://lecocorp.ams3.digitaloceanspaces.com/wp-content/uploads/20240102153310/quadjet-sd-mass-spectrometer.jpg)
Flame Ionization Detection meets GCxGC
![Pegasus BT4D with Paradigm Modulator](https://lecocorp.ams3.digitaloceanspaces.com/wp-content/uploads/20240102152115/leco-pegasus-paradigm.jpg)
Improve GC peak capacity without sacrificing accuracy
Gain more insight with this exciting resource
Benefits of GCxGC for a Routine Laboratory
Explore this resourceApplication Notes
Learn more about our solutions for GCxGC with our library of related application notes.
209-200-172 | Dual-Detection of Plastics-Derived Pyrolysis Oil Data from a single injection of plastics-derived pyrolysis oil using GCxGC with reverse fill-flush modulator and flow splitter is explored. |
209-200-173 | Reformulation of Perfume Samples with GCxGC-TOFMS/FID Chromatograms of perfume sample obtained with use of reverse fill-flush modulator and flow splitter are analyzed |
209-200-175 | Simple Group-type Analyses of Aviation Fuels Flow modulation provides high accuracy group-type analyses for traditional fuels and emerging alternatives. |
209-200-174 | GCxGC Coelution Data Detection with RFF Modulator Data demonstrating how Paradigm reverse-fill flush modulator and Shift flow splitter enhance GCxGC detection accuracy in fragrance samples. |
203-821-667 | Aroma Compounds Pumpernickel Bread Aroma Compounds Pumpernickel Bread |
News or Stories
This year marks the 20th annual conference of the Metabolomics Society! LECO is excited to be a sponsor for Metabolomics 2024, starting on 16 June and ending 20 June, and we’re counting down the minutes until the conference begins. Held in the buzzing metropolis of Osaka, Japan, this event is the largest metabolomics meeting worldwide. […]
LECO, in partnership with The Analytical Scientist, is pleased to announce the winners of our Application Challenge! Learn more about novel and interesting applications of GC-MS through interviews with the winners.
Overwhelmed by the volume of data produced by GCxGC-TOFMS? LECO’s ChromaTOF software offers data analysis add-ons that push GCxGC-TOFMS capabilities beyond what was previously feasible.
Videos and Webinars
- 1Insights from Liege: Applying Non-Target GCxGC to Enhance Metabolomics Analyses From 27 June 2024
- 2What’s in the Water? Uncovering Unknown Disinfection By-Products using GCxGC and HRMS From 31 May 2024
- 3State of the Art Technology – Aroma, Flavour, and Fragrance From 26 September 2023
- 4Discovery Workflow Best Practices for Environmental Contaminants From 30 November 2022
- 5GCxGC Analysis Workflow with ChromaTOF Tile From 30 November 2022
- 6Fast GC/Hydrogen Method Transfer – Overview and Examples From 30 November 2022
Posters and Presentations
- POPs in Dust Using MMS GCxGC-HRT
- Comprehensive Characterization of Household Dust Samples Using a Multi-Mode Source, Enhanced Chromatographic Resolution, and High-Resolution Mass Spectrometry
- Evaluation of HR-TOFMS with the Multi-Mode Ion Source and the Automated Direct Inlet Probe
- Fisher ratio analysis for metabolite discovery in Pacu fish Tile-based Fisher ratio (F-ratio) analysis is used to discover and quantify metabolites in Argentinian pacu fish, using data collected by comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GCxGC-TOFMS) via a LECO Pegasus BT 4D instrument.
- Implementation of Fisher Ratio Analysis to Improve Classification of Sulfur-Contaminated Jet Fuels using Comprehensive Two-Dimensional Gas Chromatography Time-of-Flight Mass Spectrometry Data Tile-based Fisher ratio (F-ratio) analysis is a supervised chemometric method for the discovery of analytes in comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOFMS) datasets.