GCxGC (“GC by GC”), also known as Comprehensive Two-Dimensional Gas Chromatography, is a powerful analytic technique that utilizes two columns of differing phase selectivity connected by a modulation device. The set-up of GCxGC improves peak capacity, resolution, and detectability. On average, a GCxGC analysis has five times the sensitivity and gains three times the number of compound identifications of typical GC-MS runs.
As a technique that has been around for over 25 years, some may argue that the technique has been over-hyped and a complicated research tool that doesn’t fit into routine analytical laboratories. However, several routine validated GCxGC methods have demonstrated that the technique is accurate, precise, and robust. Research has shown the benefits of GCxGC in a wide variety of applications (e.g. environmental, metabolomics, petroleum, food safety, fragrance). The benefits include increased peak capacity (i.e. resolution), structured two-dimensional chromatograms (i.e. contour plots), and sensitivity enhancement.
The merits of GCxGC as a separation science are clear, but how will it benefit a routine laboratory? Multiple analyte classes can be combined into a single analysis to save instrument and sample preparation time. Manual review time for non-target screening methods can be reduced with the increased peak resolution of a GCxGC analysis by creating a better library match leading to faster, more confident peak identification. Sample characterization is improved with a GCxGC analysis which increases confidence in decisions based on analytical results. The extra resolution afforded by the GCxGC analysis allows the use of more economical detectors, like the flame ionization detector (FID) or electron capture detector (ECD).
Joining us from William and Mary, Dr. Katelynn A. Perrault Uptmor, PI of the Nontargeted Separations Laboratory , present her work of screening samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GCXGC-TOFMS) in forensic applications as novel types of evidence appear. Dr. Perrault Uptmor highlights initial approaches to method development for new samples and show recent applications related to chemical profiling of deposited fingermarks, organic gunshot residue and decomposition odor. She also discusses the implications of GCXGC-TOFMS sample visualization in the context of expert testimony for communicating screening processes in forensic casework within the courtroom.
Part of the 2024 Non-Target Screen Webinar Series
Improve GC peak capacity without sacrificing accuracy
Explore Paradigm Flow Modulator and Shift Flow Splitter
Learn more with our latest GCxGC application notes.
Data from a single injection of plastics-derived pyrolysis oil using GCxGC with reverse fill-flush modulator and flow splitter is explored.
Chromatograms of perfume sample obtained with use of reverse fill-flush modulator and flow splitter are analyzed
Flow modulation provides high accuracy group-type analyses for traditional fuels and emerging alternatives.
Data demonstrating how Paradigm reverse-fill flush modulator and Shift flow splitter enhance GCxGC detection accuracy in fragrance samples.
Aroma Compounds Pumpernickel Bread
Watch our most recent videos on GCxGC.
Joining us from William and Mary, Dr. Katelynn A. Perrault Uptmor, PI of the Nontargeted Separations Laboratory , present her work of screening samples using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometry (GCXGC-TOFMS) in forensic applications as novel types of evidence appear. Dr. Perrault Uptmor highlights initial approaches to method development for new samples and show recent applications related to chemical profiling of deposited fingermarks, organic gunshot residue and decomposition odor. She also discusses the implications of GCXGC-TOFMS sample visualization in the context of expert testimony for communicating screening processes in forensic casework within the courtroom.
Part of the 2024 Non-Target Screen Webinar Series
Joining us from The Open University, UK, Dr. Geraint Huw Morgan, STEM Research Infrastructure Lead and Director of the Centre for Analytical Solutions, present an in-depth exploration of non-targeted screening techniques for complex aroma profiles. Drawing on his extensive experience in developing advanced methods for volatile organic compounds (VOCs), Dr. Morgan highlights innovative approaches and methodologies using advanced technologies like GCxGC-MS, and TOF-MS.
Part of the 2024 Non-Target Screen Webinar Series
Joining us from University of Liège in Belgium, Jef Focant and PH Stefanuto present how the Organic Biological Analytical Chemistry Group utilizes GCxGC-TOFMS in their research. Focant provides a comprehensive overview of testing complex matrices samples, demonstrating the wealth of information that can be gained from analyses across various metabolomics applications. Stefanuto explores the microbiome, offering insight into the using GCxGC-TOFMS to unravel complex samples, paving the way for innovative discoveries.
Part of the 2024 Non-Target Screen Webinar Series
