GCxGC (“GC by GC”), also known as Comprehensive Two-Dimensional Gas Chromatography, is a powerful analytic technique that utilizes two columns of differing phase selectivity connected by a modulation device. The set-up of GCxGC improves peak capacity, resolution, and detectability. On average, a GCxGC analysis has five times the sensitivity and gains three times the number of compound identifications of typical GC-MS runs.

As a technique that has been around for over 25 years, some may argue that the technique has been over-hyped and a complicated research tool that doesn’t fit into routine analytical laboratories. However, several routine validated GCxGC methods have demonstrated that the technique is accurate, precise, and robust. Research has shown the benefits of GCxGC in a wide variety of applications (e.g. environmental, metabolomics, petroleum, food safety, fragrance). The benefits include increased peak capacity (i.e. resolution), structured two-dimensional chromatograms (i.e. contour plots), and sensitivity enhancement.

The merits of GCxGC as a separation science are clear, but how will it benefit a routine laboratory? Multiple analyte classes can be combined into a single analysis to save instrument and sample preparation time. Manual review time for non-target screening methods can be reduced with the increased peak resolution of a GCxGC analysis by creating a better library match leading to faster, more confident peak identification. Sample characterization is improved with a GCxGC analysis which increases confidence in decisions based on analytical results. The extra resolution afforded by the GCxGC analysis allows the use of more economical detectors, like the flame ionization detector (FID) or electron capture detector (ECD).

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Instrument Portfolio

Pegasus Two-Dimensional Gas Chromatograph
Pegasus BT 4D

GCxGC Time-of-Flight Mass Spectrometer

Pegasus High Resolution two-dimensional gas chromatograph
Pegasus GC-HRT 4D

High Resolution GCxGC-TOFMS

QuadJet Mass Spectrometer
QuadJet SD

Flame Ionization Detection meets GCxGC

Pegasus BT4D with Paradigm Modulator
Paradigm Flow Modulator and Shift Flow Splitter

Improve GC peak capacity without sacrificing accuracy

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Benefits of GCxGC for a Routine Laboratory

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Application Notes

Learn more about our solutions for GCxGC with our library of related application notes.

News or Stories

30 June 2023 Excellence in GC-MS Applications

LECO, in partnership with The Analytical Scientist, is pleased to announce the winners of our Application Challenge! Learn more about novel and interesting applications of GC-MS through interviews with the winners.

9 June 2023 GCxGC Data Handling: ChromaTOF Tile

Overwhelmed by the volume of data produced by GCxGC-TOFMS? LECO’s ChromaTOF software offers data analysis add-ons that push GCxGC-TOFMS capabilities beyond what was previously feasible.

19 May 2023 Global Mass Spectrometry Applications Meeting

The global voice of our customer base was reflected back at us during our international Mass Spectrometry team meeting. Let us hear your own thoughts at GCxGC, ASMS, or the Metabolomics Conference later this year!

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